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Data were analyzed by methods recently described by Biegel et al. (1998) to evaluate THF-diol and/or LTX-diol effects on the estrous cycle in rats.
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The assays described here as well as complementary methods recently described by others [69], [70] open up exciting future opportunities for the elucidation of the possible role of mitochondria and mtDNA in this context.
Two types of AuNPs suspensions were compared: AuNPs obtained via the well-known Turkevich method, citrate-stabilized and having a diameter of about 20 nm, and AuNPs obtained by the method recently described by Eah et al., stabilizer-free with an average diameter of 4 nm.
Such a diversity, which is expected using this technique, could probably be improved by the method recently described by Goletz et al (2002), which involves specific elution with the antigen followed by trypsin treatment of eluted phages for generating large diversities of anti-Id single-chain antibody fragments from nonimmunised phagemid libraries using phage display.
This is done by a numerical method recently described [ 50].
A gradient of concentrations of acetonitrile and aqueous solution of ammonium acetate was created according to the method recently described by Almqvist et al. (2016).
A similar method recently described by Varkonyi-Gasic et al. [22] also showed high specificity and sensitivity.
ASE when available on a high-throughput platform, similar to the method recently described by Serre et al [1], may have significant advantages over total gene expression data as it is a cleaner representation of cis-acting effects.
To further investigate possible role of E6 and E7 oncoproteins in the metastatic process the two oncoproteins were knocked-down (KD) in HeLa cells using a method recently described by Biard [30].
Our approach resembles the method recently described by Scott et al. [ 22], although it is unclear how unapplicable items were taken into account in their study.
Fluorescence lifetime measurements were made using the phasor FLIM method recently described [46] [48].
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