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Fig. 5 Fed-batch biotransformation of FA. C. glutamicum strains FVan (closed symbols) and F (open symbols) were cultivated in BY medium in a flask for 24 h, collected, and inoculated into a bioreactor.
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It was shown that both the pendant vinyl groups and Py molecules are affected by the reaction medium in a similar way, their mobility decreases as the DVB concentration increases or the temperature decreases.
Trypsin was inhibited by addition of fresh medium in a 5 : 1 volume ratio; cells were centrifuged at 800 ×g for 5 minutes and further plated to obtain 7 to 10 times area dilution.
Transformants were selected by growth on kanamycin-containing medium in a 5% CO2-enriched atmosphere.
Assemblies can grow by incorporating molecules from the medium in a catalyzed (fast) and uncatalyzed (slow) manner.
The cells were then transferred to fresh LB medium in a flask by 1 100 dilutions, and cultured at 37°C and 250 rpm.
Survival and calcium signaling of osteoclasts are significantly enhanced by acidification of the medium in an OGR1-depedent manner [22].
PCR products were separated by capillary electrophoresis through a polyacrylamide medium in an ABI 3730 genetic analyzer.
Imbued with realistic touches, alive with filigree details, and emitting a translucence bequeathed by the medium in the hands of a master, the series "Transparent Strokes" was snapped up by visitors to the city.
Acetic acid was discovered in the interstellar medium in 1996 by a team led by David Mehringer who detected it using the former Berkeley-Illinois-Maryland Berkeley-Illinois-Maryland Berkeley-Illinois-Maryland Associationer Millimeter array located atheHatOwens Valley Radio Observatory.
The prominent stimulation of DNA synthesis in primary HUVECs by whole ASP13 conditioned medium, and in a less conspicuous manner by CYS12 supernatants, propose significant paracrine effects of tumour cell-derived VEGF-A in neovascularization [ 34].
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