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To investigate this, we compared the proliferative status of DN γδ thymocytes in WT γδTCR Tg, Lck+/− γδTCR Tg, and Fyn+/− γδTCR Tg mice by measuring their expression of the Ki-67 Ag, which is a marker of actively cycling cells [34], [35].
Further, we validated these miRNAs by measuring their expression in tumors tissues and adjacent liver tissues.
Although the set of active miRNAs can often be determined experimentally (by measuring their expression levels), identifying their targets is much more challenging.
2) Is there a particular group of genes regulated by miRNAs, which are differentially expressed between species and/or tissues? 3) Can the functional relationship between miRNAs and their target genes be determined by measuring their expression simultaneously?
In relation to the role of IL-7 in Th1 polarization, the commitment of cells towards Th1 was further investigated by measuring their expression of TBET (TBX21) and GATA3, two transcription factors essential for Th1 and Th2 polarization, respectively [ 45].
The phenotype of the cells was characterised by measuring their expression of VE-cadherin/CD144 and VEGF receptor 2 (VEGFR2), and the functionality assessed through the ability of the cells to form tubules on matrigel.
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To investigate this possibility, we monitored the temporal interaction between BAT1 and APN by measuring their co-expression in oocytes over 6 days.
To confirm the reproducibility of miRNA expression profiles examined by miRNA array analysis, we measured their expression levels by RT-PCR using a miRNA reverse transcription kit and qPCR detection kit (Genecopoeia, Germantown, MD, USA).
To examine the regulation of these genes by N-Myc, we measured their expression using neuroblastoma cells that contain a Tet-regulatable N-myc transgene (TET21N) as well as NSC with a nestin-cre driven N-myc knockout.
Because levels of Foxp3+ and CTLA-4 expression in Tregs have been correlated with suppressor function of these cells and these antigens are also known to be up-regulated by Treg activation, we measured their expression in freshly isolated and exogenously expanded Tregs from young and old B/W mice by FACS.
We expressed these chimeric proteins in T cells of the CEM line by transient transfection, and then measured their expression at the cell surface using flow cytometry [Figure 3 A, which shows the relative cell number vs. CD8 staining intensity (phycoerythrin) for the mid to low intensity GFP-positive (transfected) cells].
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by measuring their fluorescence
by measuring their heart
by measuring their retention
by measuring their extinction
by measuring their association
by measuring their absorbance
by measuring their water
by measuring their attenuation
by measuring their finger
by measuring their concentration
by measuring their collapse
by measuring their prevalence
by measuring their effect
by measuring their surface
by measuring their impact
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