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Additionally, the VP16 activator induced a significantly higher degree of chromatin decondensation than the p53-activator, as determined by measuring the pixel area of the transcription site (Fig. 9C, panels a, d and g, and 9F).
The quantitative analysis image was performed using the imaging software Image J. The relative distribution of p27kip1 protein between the two compartments nucleo/cytoplasm, was calculated by measuring the pixel average signal both in the nucleus, cytoplasm and nucleo/cytoplasm compartments.
The relative cell surface expression of Cntn4, -5 and -6 was analyzed by measuring the pixel intensities of images from a confocal-like microscopy technique.
Western blot data acquisition and analysis were accomplished by measuring the pixel density and area of immunoreactive bands from the ECL films using the ImageJ software (NIH).
Images of gels were further analysed using ImageJ (http://rsbweb.nih.gov/ij/), whereby relative DNA band intensities were semi-quantitatively assessed by measuring the pixel intensity of nuclease-treated samples compared against untreated controls.
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Images were quantified by measuring the mean pixel intensity using ImageJ (NIH).
Fluorescence signal was quantified by measuring the mean pixel value of a manually selected somatic area for each frame of the image stack using ImageJ software (http://rsb.info.nih.gov/ij/).
Because of low and variable transfection efficiencies this could not be assayed at a population level; thus transfected cells were stained with the antibodies and the levels of the phosphorylated pRb in the nucleoplasm were quantified by measuring the average pixel intensity (Figure 4B).
Quantitative analysis was performed using Zen software (Carl Zeiss, Jena, Germany) by measuring the average pixel intensity for each image.
Green fluorescence was quantified by measuring the medium pixel intensity in the confocal images for every single nodule analyzed.
Semi-quantitative analysis of MSK2 expression in human embryos was performed by measuring the mean pixel intensity of immunostaining in each blastomere using ImageJ.
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by finding the pixel
by permuting the pixel
by determining the pixel
by thresholding the pixel
by summing the pixel
by fitting the pixel
by investigating the pixel
by concatenating the pixel
by averaging the pixel
by using the pixel
by multiplying the pixel
by examining the pixel
by reducing the pixel
by considering the pixel
by computing the pixel
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