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By measuring the evolutionary importance of the link between residues,, we could then infer the importance of i from the average of over all its neighbors j.
We confirmed the difference in selection constraint at NRs and NDRs by measuring the evolutionary rates of TFBSs at these regions Moreover, observations reported in literature support our findings by demonstrating the differences in the accessibility of DNA to their binding proteins inside and outside nucleosome occupied regions [ 60, 62, 72].
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CAPS 2.0 (Coevolution analysis using protein sequences; http://bioinf.gen.tcd.ie/~faresm/software/software.html [ 34]) with default parameters was used to identify co-evolving amino acid site pairs (e and k) by measuring the correlated evolutionary variation at these sites.
Furthermore, these studies do not infer evolvability by measuring the amount of evolutionary change brought about through positive selection, as we do here.
This finding was confirmed by measuring the difference in evolutionary rates of binding sites in sensu stricto yeasts at nucleosome occupied regions and nucleosome depleted regions.
Instead, we determine which species has an evolutionary advantage by measuring the resistance of bacteria to their sympatric phages: greater evolutionary potential of phages should result in lower sympatric resistance.
So, at least, in principle, by measuring the extent of "homogenization" of evolutionary rates of domains in multidomain proteins, it should be possible to disentangle and compare the contributions of translation rate-mediated factors and translation-independent, intrinsic ones.
We further assessed the importance of the U-tracts in Alu elements by performing an evolutionary analysis measuring the strength of selection.
Species-richness can be assessed by measuring phylogenetic diversity -- the total evolutionary history shared across all species within a community.
Another recent study by [ 10] has also measured the coherency of evolutionary conservation of interaction patterns in an integrated network of S. cerevisiae comprising transcriptional and protein-protein interaction data.
Quantifying evolutionary rates by measuring change in average phenotype through time can potentially oversimplify inferences of evolutionary trends [ 35– 39], as apparent trends can arise from changes in the mean and/or the variance of the distribution of trait values [ 35, 40– 40].
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