Sentence examples for by measuring the binding from inspiring English sources
GAPDH and β actin were used as internal controls, and the detection was carried out by measuring the binding of fluorescence dye SYBR green to double-stranded DNA.
(B) The immunoreactivity of 125I-labeled B-B4 mAb was estimated by measuring the binding of a constant concentration of 125I-labeled B-B4 mAb to increasing numbers of U266 multiple myeloma cells that strongly expressed the CD138 antigen.
A key step in the initiation of the intrinsic apoptotic pathway is the oxidation of cardiolipin, which is assessed in this study by measuring the binding of the fluorescent compound NAO through flow cytometry.
Classical evaluation of target selectivity is usually undertaken by measuring the binding affinity of lead compounds against a number of potential targets under equilibrium conditions, without considering the kinetics of the ligand receptor interaction.
Next, we examined NA antigenicity by measuring the binding affinity of anti-NA mAbs to the mutant viruses.
We began by measuring the binding affinity (Kd) of each protein for ATP by equilibrium spin-filtration [16].
A comparative analysis is reported for testing solutions in the presence and absence of NTA by measuring the calcium binding affinity of fluorescent cardiac troponin C.
After defining Influenza A/Puerto Rico/8/34 Matrix Protein M1 (H1MP) 40–54 as a DR1201 restricted epitope, the critical anchor residues within this sequence were confirmed by measuring the relative binding of peptides with non-conservative substitutions in competition with biotin labeled H1MP40 54 peptide.
The expression of hDOP G-protein fusions was assessed by measuring thDOP G-proteinnding ofusionsprenorphine in cell-membrane preparations.
The nociceptin receptor fused with the G protein alpha subunit (Giα), whose ligand recognition can be detected by measuring the [S]GTPγS binding, was also successfully expressed in the same system as the nonfused protein as described above.
Gene product imaging can be accomplished by measuring the accumulation or binding of the respective radiolabeled substrates or ligands.
