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The anti-inflammatory properties of CEN1 HC-Br, CEN1 HC, CEN1 HC (1 20) and CEN1 HC (Ser) were evaluated by measuring secretion of the most commonly used inflammation markers TNF-α and IL-6 in human monocyte derived macrophages stimulated with LPS.
Biological effects of chemerin21-157 were investigated by measuring secretion of pro-inflammatory cytokines and metalloproteases in cell supernatants.
We further analyzed A β neurotoxicity by measuring secretion of the pro-inflammatory cytokine IL-1 β and lactate dehydrogenase (LDH) release in the culture media of NLRP1 siRNA- and control siRNA-transfected cells.
Human umbilical vein endothelial cell (HUVEC) activation was monitored for 15 hours by measuring secretion of IL-6 and IL-8, as well as surface expression of the adhesion molecules ICAM-1 and E-selectin.
Lentivirus-infected cells were selected with puromycin (2 µg/ml)), and specific knock-down of the gene of interest was evaluated by measuring secretion of decorin in 24 h-conditionned medium.
Endothelial cell activation was monitored for up to 15 hours by measuring secretion of IL-6 and IL-8, as well as surface expression of the adhesion molecules ICAM-1 and E-selectin.
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This loss-of-function effect was confirmed by measuring the secretion of endogenous mediators in supernatants of HEK 293T cells (n = 3, p<0.0001; Fig. 4C).
Thus, the clear loss-of-function effect of S183I RIG-I SNP was confirmed by measuring the secretion of IL-8 (n = 3 p<0.0001; Fig. 3D) and RANTES (not illustrated) in the supernatants of stimulated HEK 293T cells.
Moreover, the activity of specific steroidogenic reactions can be monitored by measuring the secretion of reaction substrate(s) and product(s) from in vitro perfused testes.
A proinflammatory response was assessed by measuring the secretion of the pro-inflammatory cytokine interleukin 8 (IL-8) with an ELISA assay (kit from Invitrogen).
The effect of various blocking antibodies and inhibitors on cell toxicity was determined by measuring the secretion of lactate dehydrogenase (LDH) using the Cytotoxicity Detection Kit (Roche Diagnostics, Mannheim, Germany) according to manufacturer's recommendation.
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