Sentence examples for by measuring mRNA levels from inspiring English sources

Quantitative real time-polymerase chain reaction (qRT-PCR) was used to determine the effects of all treatments on the relative expression of target genes by measuring mRNA levels.

In addition, we validated our microarray data by measuring mRNA levels of representative genes (Scd2, Srebf1, Lpin1, Pparg, Cyp7a1, and Abcb11) using quantitative real time-PCR (Figure S2 and S3).

Identification of miRNA targets was assessed using luciferase assays and by measuring mRNA levels.

Loss of epithelial cells was further investigated by measuring mRNA levels of the epithelial marker villin (figures 2E and 3C).

We also evaluated tubular damage by measuring mRNA levels of the representative nephrotoxicity biomarkers NGAL and KIM-1.

In general, accurate up-front knowledge of expression levels is most easily achieved by measuring mRNA levels.

To determine if the transcription activity of Sp1 was affected by SENP3, we measured mRNA levels of several target genes of Sp1.

To investigate whether the observed selective changes in muscle sphingolipid species abundance were dictated by changes in enzyme expression, we measured mRNA levels of a range of these by quantitative PCR.

Previous studies using animal pain models have measured mRNA levels by examining a targeted set of transcripts or through the use of global approaches such as microarray technology to study mRNA expression changes [5], [6].

Pain model studies have measured mRNA levels by either looking at a targeted set of transcripts or through the use of global approaches such as microarray technology to study changes in neuropathic pain.

To more accurately quantitate gene expression, we isolated spontaneous tumors from the C3(1) TAg and MMTV-Neu models and measured mRNA levels by quantitative reverse transcription and polymerase chain reaction (qRT-PCR; Figure 1c, left panel).