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We confirmed that these cells were under ER stress by measuring expression levels of typical ER stress markers, spliced XBP-1 and BiP.
hESC differentiation was determined by measuring expression levels of NESTIN and PAX6 for ectoderm, T and DESMINE for mesoderm and GATA4 and SOX17 for endoderm.
To determine whether Wnt signaling plays a role in prostatic tumorigenesis and tumor progression, we assessed the Wnt signaling status in human prostate cancer cell lines and 2 human prostate tumor xenografts by measuring expression levels of Axin2.
The generation of cells of the pancreatic lineage was assessed by measuring expression levels of the following markers: IAPP, IGRP, insulin I and insulin II (beta cell markers), the transcriptional regulators Nkx 6.1 and PDX-1 (pancreas development markers) and an endocrine marker, PAX 6 using Q-PCR.
The response was further confirmed by measuring expression levels of known independent endogenous genes TXNIP[ 5] and ANKRD11[ 34].
Here, we confirmed immune system activation after LPS administration by measuring expression levels of an immune response gene (Defensin2) in the fat bodies of honey bee workers.
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To determine whether PERK signaling is involved in WFS1 upregulation by valproate, we measured expression levels of phosphorylated eIF2α, which reflect PERK activation levels.
CAGE is a high-throughput method to measure expression levels by counting large amounts of sequenced capped 5' ends of transcripts, termed CAGE tags [15], [16].
We further confirmed the effect of EP1 and EP3 on uPA, MMP-2, and MMP-9 expression by measuring the expression levels of uPA, MMP-2, and MMP-9 after blocking EP1 and EP3 expression with gene-specific siRNAs.
Since our array experiment and QRT-PCR assays were designed to measure the levels of only mature miRs, we also investigated whether the TWEAK regulates the differential expression of these miRNAs at transcriptional level or at post-transcriptional level by measuring the expression levels of their processing enzymes using QRT-PCR assays.
By measuring the expression levels of Dlk1 in muscle-specific knock-out mice, it was observed that Dlk1 levels were only decreased by 35% in whole muscle compared to wild-type siblings.
More suggestions(15)
by analyzing expression levels
by measuring mercury levels
by measuring protein levels
by dividing expression levels
by measuring expression changes
by regulating expression levels
by measuring mRNA levels
by measuring blood levels
by measuring energy levels
by measuring hepcidin levels
by balancing expression levels
by measuring dopamine levels
by increasing expression levels
by measuring cytokine levels
by measuring water levels
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