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Polymorphonuclear neutrophil activation was analyzed by measuring cell surface and soluble cell adhesion molecule expressions.
We assessed class-switching of atypical MBCs by measuring cell surface expression of IgG by flow cytometry (Fig. 4).
We provide evidence that cells sense a critical cell size by measuring cell surface area rather than, for example, cell volume or absolute length, a mechanism that could function regardless of the cell shape.
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Since cell roundness appears to correlate with chondrocytic behavior of dermal fibroblasts, this study quantified roundness by measuring cell height and surface area volume ratio.
Response to the different films was evaluated by measuring cell adherence to the surface along with proliferation.
In the degranulation assay, the cytotoxicity of NK cells was determined by measuring the cell surface expression of the lysosomal marker, CD107a, which correlates with NK cell cytotoxicity [ 19].
Cell growth was analyzed by measuring the cell surface area with an in-house macro and ImageJ software (NIH, Bethesda, MD).
ATM activation did not trigger the differentiation of the monocytes into dendritic cells or macrophages, as verified by measuring the cell surface expression of CD1a, CD14, CD80 and CD86.
This was done by measuring the cell surface expression of mesothelin in NCI-H226 tumor spheroids by flow cytometry.
Leukocyte activation was investigated by measuring the cell surface expression of CD62L (L-selectin), CD11b (Mac-1) and the intracellular production of hydrogen peroxide (H2O2), via flow cytometry.
Cell surface areas were determined by defining cyanobacteria cells as spherical or cylindrical, then measuring cell diameter (all cells) and length (idealised cylindrical cells).
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