Sentence examples for by measuring binding of from inspiring English sources
Online substrate cleavage was monitored by measuring binding of mAb10F12 to a SNAP-25 neo-epitope.
The proper folding of yeast-displayed CXC chemokines was assessed by measuring binding of some displayed CXC chemokines to a panel of commercial neutralizing antibodies (Supplementary Table 13).
Joshi et al. [5] tried to address this problem by measuring binding of phospholipidosis-inducing drugs to L-α-dipalmitoyl phosphatidylcholine vesicles.
The immunoreactivity was determined by measuring binding of Zr-cU36, Zr-cetuximab, or Zr-rituximab (10,000 cpm/ml) to a serial dilution of 0.2% glutaraldehyde-fixed 11B cells or 2% paraformaldehyde-fixed A431, or Ramos cells, respectively, essentially as described by Lindmo et al. [ 19].
These mAb were analyzed for their fine specificity by measuring binding to synthetic analogs of the decapeptide, and cDNA sequences encoding the mAb V regions were determined.
We first examined AECA activity in the sera from patients with collagen diseases by measuring binding activity of IgG to nonpermeabilized 7-AAD-negative HUVECs by using flow cytometry.
Therefore, we decided to probe for thermodynamic synergy between pairs of ESS3b residues by measuring binding affinities using double-cytosine-substituted SL3ESS3 constructs.
Typical applications involve monitoring the amplification products indirectly, such as by measuring binding-dependent fluorescence of specific dyes (e.g., SYBR Green).
The K472E/R473E mutant Cul1 was then used to measure binding of CAND1 by coimmunoprecipitation.
Pioneering studies have demonstrated that chromatin immuno-precipitation followed by deep sequencing (ChIP-seq) can reliably measure binding of P300, a histone acetyltransferase and transcription activator, across the genome to predictively map transcriptional enhancer elements active in vivo [ 5– 7].
The activation state of endogenous CHO cell α5β1 was assessed by measuring the binding of a recombinant soluble integrin-binding fragment of fibronectin (FN9 11) in three-colour flow cytometric assays as described earlier (Tadokoro et al, 2003; Calderwood et al, 2004).
