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This paper discusses some preconditions for "making things the same" by means of quantification and economic calculation.
Monitoring of response was performed after each treatment cycle and at study-end by means of quantification of serum immunoglobulins, serum protein electrophoresis, immunofixation and collection of 24-hr urine specimen for total protein, electrophoresis and immunofixation.
The third paper, that reported changes of the microbiota related to interference in the vitamin D system, assessed susceptibility to DSS colitis in conditional VDR KO mice (deletion restricted to the intestinal epithelial cells), along with Paneth cell quantity and quality by means of quantification of lysozyme and ATG16L1 protein expression.
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The number of adherent cells was determined by means of nuclei quantification on the TiO2-nano, Ti-nano, and Ti-micro substrates [34].
Further analysis of the recurrence plot of squealing events by means of recurrence quantification analysis measures reveals different regimes of laminar and random behaviour, periodicity and chaos-forming recurrent transitions.
Amounts of copies/mL in each sample were determined by means of a quantification software (Amplimedical), by considering an extraction recovery of 80%.
Secondary 'sieving' of patients with positive FOBTs returned in the screening round of the national colorectal cancer screening programme could be performed by means of ELISA quantification of sMMP-9.
The presence of SMARCB1/INI1 hemyzigous deletion was confirmed also by Real-time quantitative PCR analysis of SMARCB1/INI1 copy number by means of relative quantification using standard curve method (not shown).
Analysis of SMARCB1/INI1 copy number by real-time quantitative PCR was performed by means of relative quantification using standard curve method [ 32], using TaqMan assays 4401631 (Applera) for RPPH1 endogenous control gene and Hs01497967_cn (Applera) for SMARCB1/INI1.
Additional groups (control and 500 microg/m(3)) were studied by means of morphometric analyses (quantification of the amount of intraepithelial and surface mucins), measurement of cilia beat frequency, and quantification of total glutathione.
Relative gene expression was calculated by mean of relative quantification (2-ΔΔCt) as previously described [ 60].
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