Roehl haplotype data files (RDF) were created with DnaSP and imported into Network v4.5.0.1 (Fluxus-Technology, www.fluxus-engineering.com) and networks were calculated with the Median-Joining algorithm using maximum parsimony post-processing [35].
The MJ network was maximum parsimony post-processed to display the shortest tree compatible with the predictions of coalescence [ 98].
Tree-building was a two-step process: A preliminary input tree was first constructed by maximum parsimony in PAUP* 4.0b10 [51].
Phylogenetic analysis of six Halicephalobus sequences and four outgroup taxa by maximum parsimony yielded one tree with five well-supported clades.
Construction of phylogenetic trees and divergence analysis was performed by maximum parsimony method using MEGA 4 software (Molecular Evolutionary Genetics Analysis) [26].
Phylogenetic trees were constructed by maximum parsimony method from aligned sequences or by using a maximum likelihood method from the best model identified by Modeltest 3.7.
Phylogenetic trees were obtained by maximum parsimony (MP), maximum likelihood (ML), and Bayesian methods.
Phylogenetic analysis by maximum parsimony was obtained by the heuristic search method.
The phylogenetic analysis was performed by maximum parsimony method using MEGA [ 84].
The alignments were analysed by maximum parsimony (MP) and a Bayesian approach (B/MCMC) [ 85, 86].
