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Construction of phylogenetic trees and divergence analysis was performed by maximum parsimony method using MEGA 4 software (Molecular Evolutionary Genetics Analysis) [26].
Phylogenetic trees were constructed by maximum parsimony method from aligned sequences or by using a maximum likelihood method from the best model identified by Modeltest 3.7.
The phylogenetic analysis was performed by maximum parsimony method using MEGA [ 84].
The phylogenetic tree constructed by maximum parsimony method is in agreement with our grouping based on motif conservation.
Human known miRNAs were thus classified into eight intervals (groups) based on their evolutionary ages estimated by maximum parsimony method.
The tree constructed by the neighbor-joining method is highly similar to that produced by maximum parsimony method (data not shown).
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Phylogenetic relationship of various strains was estimated by the maximum parsimony method by assuming that both base change and indel are treated as a single event.
The resulting topology was nested into the general orchid tree build by a maximum parsimony method by Górniak et al. (2010).
Figure 4 Phylogenetic tree based on tuf gene sequences of ChIF phytoplasma and related 17 phytoplasmas, constructed by the maximum parsimony method.
In this analysis, we used a maximum likelihood method to reconstruct the evolution of 2,961 introns in a dataset of 76 ribosomal protein genes from 22 eukaryotes and validated the results by a maximum parsimony method.
The seven housekeeping alleles were concatenated (3,134 nucleotide sites) for each of the 80 STs of SDSE, and the concatenates used to construct a phylogenetic tree by the maximum parsimony method (Figure 3).
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