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The robustness of tree topologies was confirmed by maximum parsimony analysis (Ludwig et al. 2004) with 100 bootstrap replications (Felsenstein 1981).
Construction of a phylogenetic tree by maximum parsimony analysis illustrates the genetic proximity of isolates from this study to those collected from the northeastern United States in 1999, 2000, and 2001.
The phylogenetic tree shown in Figure 2 was constructed by maximum parsimony analysis with env gene sequences from US isolates from 1999 2006 in GenBank and sequences of human isolates in our study.
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Nucleotide sequences without primer sequences were aligned and clustered by maximum-parsimony analysis in BioNumerics version 4.6 (Applied Maths, Kortrijk, Belgium) and corresponded with analysis of a 242-nt fragment of ORF1 and a 148-nt fragment of ORF2.
The strict consensus of 6 trees yielded by MP (Maximum Parsimony) analysis (L = 1182, CI = 0.884, RI = 0.873) was generally congruent with the ML (Maximum Likelihood) tree and the majority rule BI (Bayesian Inference) tree in the topology (Additional file 1: Figure S2).
The maximum parsimony analysis by NONA yielded only one most parsimonious tree (Fig. 11a; tree length = 70 steps, consistency index (CI) = 0.60, retention index (RI) = 0.68).
On the contrary, the maximum parsimony analysis conducted by Gu and Li advocated for recent enrichment of the GC content of GC-rich genes in some genomes, e.g. the rabbit.
Monophyly of Characiformes, however, was not recovered in the two publications of Ortí and Meyer, who analyzed characiphysan phylogenies using nuclear ependymin [ 29] and mitochondrial rDNA sequences [ 19], which is also true for Peng et al. [ 30] and the results of maximum parsimony analysis reported by Saitoh et al. [ 4] but in a different manner.
Unweighted maximum parsimony analysis yielded five equally parsimonious trees.
Although analyses of whole mitogenome sequences by Saitoh et al. [ 4] (maximum parsimony analysis of 8096 bp) and Peng et al. [ 30] (6198 bp) did not include citharinoids, one of the two characoids were more closely related to either Siluriformes or Gymnotiformes than to its own order.
Maximum parsimony analysis was performed by using heuristic searches.
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