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The Δyap1801/1802 strain was constructed by mating the two simple deleted strains Δ yap1801 and Δ yap1802.
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Thus we tested whether albinism in the adult F0 animals could be transmitted to the next generation by mating the three mature frogs.
The third cross (WxM) was obtained by mating the same W boar with four Meishan (M) sows.
We created tetraploid deletion series for each of the eight genes under consideration by mating the relevant mating-competent diploid deletion mutants, as described in Methods.
The first stage followed a half-diallele mating system by inter-mating the eight founder lines and 28 bi-parental crosses were made.
We investigated reproductive capacities of COUP-TFflox/flox (Tamoxifen), Cre-ERTM COUP-TFIIflox/flox (Oil), Cre-ERTM COUP-TFIIflox/+ (Tamoxifen) and Cre-ERTM COUP-TFIIflox/flox (Tamoxifen) mice by mating one male with two females for 3 months.
Two F3 crosses were established in December 2010 by mating two F2 full-sibs to create one family, and two F2 half-sibs to create the other.
After adjusting copy numbers of the above-mentioned rate-limiting enzymes to further balance the metabolic flux, a diploid strain YastD-01 was generated by mating two astaxanthin-producing haploid strains carrying the same carotenogenic pathway.
Mouse α-synuclein null and the littermate wild type mice were generated by mating two mice that are both heterozygous for the mouse α-synuclein gene.
Transcription of RNAi in spermatogonia and spermatocytes was induced by mating virgins carrying two copies of the Bam-Gal4-VP16 (Chen and McKearin, 2003) driver to males carrying the UAS constructs.
The new member is produced by mating two successful parents through a crossover routine, and then mutating that new member.
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