Choi, J. W. et al. Whole-genome analyses of Korean native and Holstein cattle breeds by massively parallel sequencing.
Freeman, J. D., Warren, R. L., Webb, J. R., Nelson, B. H. & Holt, R. A. Profiling the T-cell receptor beta-chain repertoire by massively parallel sequencing.
These complexes can be stabilized by crosslinking and ligating the crosslinked enhancer and target fragments together, and the interacting elements identified by massively parallel sequencing.
In this study, we evaluated the downstream effects of extended processing times on the reliability of aneuploidy detection by massively parallel sequencing (MPS).
Current protocols in molecular biology Christodoulou, D. C., Gorham, J. M., Kawana, M., DePalma, S. R., Herman, D. S., Wakimoto, H. 2011; Chapter 25: Unit25B 9-? Deep sequencing analysis of gene expression (DSAGE) measures global gene transcript levels from only 1 to 2 µg total RNA by massively parallel sequencing of cDNA tags.
To correlate the variable clinical features of oestrogen-receptor-positive breast cancer with somatic alterations, we studied pretreatment tumour biopsies accrued from patients in two studies of neoadjuvant aromatase inhibitor therapy by massively parallel sequencing and analysis.
The results of our study are more general than just expression profiling by massively parallel sequencing.
Hence, the comparison of gene expression between species should be possible by massively parallel sequencing.
Mutations only detected by massively parallel sequencing occurred at allele frequencies of less than 10% (Table 1 and Fig. 2A).
In complex genomes, expression profiling by massively parallel sequencing could introduce a considerable bias due to incorrectly mapped sequence reads if the read length is short.
We present the readDepth package for R, which can detect these aberrations by measuring the depth of coverage obtained by massively parallel sequencing of the genome.
