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Expression profiling by massively parallel cDNA sequencing (RNA-Seq; [ 23]) is a cost-effective way to survey transcriptomes of different tissues and developmental stages.
In this paper we analyse the parental strain DB110 and the toxR mutant TW30 by massively parallel cDNA sequencing (RNA-seq).
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In contrast, transcriptome information generated by the use of massively parallel cDNA sequencing (RNA-seq) provides absolute measures of gene expression and can quantify the levels of known and unknown genes, facilitating annotation of the genome while identifying patterns of gene expression (Mortazavi et al. 2008).
Massively parallel cDNA sequencing (RNA-seq) experiments are gradually superseding microarrays in quantitative gene expression profiling.
Massively parallel cDNA sequencing (RNA-seq) is gradually superseding microarrays in quantitative gene expression profiling [ 1].
Recent development of massively parallel cDNA sequencing (RNA-Seq) provides a more powerful and cost-effective way to analyze transcriptome data.
Thanks to the massively parallel cDNA sequencing (RNA-Seq) technologies [ 7], scientists can obtain cDNA fragments from transcriptomes with reasonably complete coverage in a reduced time scale and at a lower cost [ 8].
Recently, the rapid development of novel massively parallel cDNA sequencing, or RNA-Seq, has allowed many advances in the characterization and quantification of transcriptomes in fish, such as salmonids, whitefish, catfish and Japanese seabass.
Information obtained from analysis of the T. parva transcriptome by massively parallel signature sequencing (MPSS) technology combined with the fact that we isolated several SVSP clones from a cDNA library indicates that many SVSP genes are transcribed in the schizont stage ([25], [28] and data not shown).
The results of our study are more general than just expression profiling by massively parallel sequencing.
Hence, the comparison of gene expression between species should be possible by massively parallel sequencing.
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