Eventually, five distinct non-allelic genomic fragments exhibiting substantial homology to plant Sus genes were identified by massive sequencing of the PCR-generated clones.
Recently, such an approach proved successful by massive sequencing of reduced representation libraries of multiple grape varieties to develop a ~9,000 selected SNP array from over 470,000 in silico detected SNPs [ 13].
In addition, to provide the users with ChIP-Seq (chromatin immunoprecipitation followed by massive sequencing) data for TFs of the ENCODE project, we also integrated a clickable button linked to these tracks, loaded into an open-access custom session of UCSC Genome Browser.
The integration of the functional analyses provided by the massive sequencing of bacterial genomes and metagenomes will allow the rational production of a desired prebiotic molecule with specific functional properties.
First, integrative metagenomic studies - aiming at the simultaneous examination (or even better at the integration) of observations about the elements, functions and evolutionary processes captured by the massive sequencing of multiple markers - should be preferred over DNA barcoding projects and over metagenomic projects based on a single marker.
In order to determine the whole NtcA regulon, we attempted to find out all the NtcA targets present in the genome of Anabaena sp. PCC 7120 using immunoprecipitation of chromatin followed by massive sequencing (ChIP-Seq).
Neiman, M., Lundin, S., Savolainen, P. & Ahmadian, A. Decoding a substantial set of samples in parallel by massive sequencing.
miR-3151, which was first identified by massive sequencing techniques, is located within the first intron of the BAALC gene.
This level of genomic variability is unexpected considering the difficulty of distinguishing among strains using standard molecular techniques and underscores the resolution provided by massive sequencing techniques.
Sequencing-by-ligation (SBL) is one of several next-generation sequencing methods that has been developed for massive sequencing of DNA immobilized on arrayed beads (or other clonal amplicons).
This project consists of massive sequencing of the complete genome of hundreds of individuals from different populations worldwide by using massive sequencing platforms, with the final goal of providing information about the overall human genetic variability, including low frequency polymorphisms in different human populations.
