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To exclude the possibility that hitherto-undetected cryptic origins might have been activated in the mutant backgrounds, we determined the genome-wide replication profile of the WT and Δ orc1-1/Δ orc1-3 mutant strains by marker frequency analysis (MFA) in conjunction with next-generation sequencing.
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Three origins were mapped in Sulfolobus solfataricus and Sulfolobus acidocaldarius by means of microarray-based marker frequency analysis (MFA) [ 6], and two origins adjacent to the orc/cdc6 genes were previously identified by two-dimensional gel electrophoresis [ 5].
Indeed, we found that replication initiation was largely unaffected by ScpA cleavage at early stages of spore outgrowth, as judged with marker frequency analysis.
To address whether or not the mapped ORC1/CDC6 binding sites correspond with replication origins, we employed marker frequency analysis (MFA).
Convergence was verified by frequency analysis.
The data will be described by means of frequency analysis.
In the trigenomic hybrid, homologous chromosome pairs Aj-An, Bj-Bc and Cn-Cc had similar meiotic crossover frequencies and segregation to that previously observed in established Brassica species, as demonstrated by marker haplotype analysis of the MD population.
Our study also indicates that haplotype-based analysis can be much more powerful than marker-by-marker analysis.
Frequency analysis was performed by the chi-square test.
"Frequency Analysis of Extreme Events". In Handbook of Hydrology, edited by D. Maidment.
Frequency Analysis of English Usage.
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