Sentence examples for by mapping a read-pair from inspiring English sources
By mapping a read-pair to a long edge we can compute its insert size and thus classify read-pairs with abnormally large insert sizes as chimeric.
The mean coverage obtained by mapping read pairs with the correct fragment size was 82.9× and we used a minimum coverage of 20-fold to call changes (see Section 2).
Of these events, 1,410 were indicated by a single uniquely mapping read-pair (table 2).
Briefly, DB uses the Binary Alignment/Map (BAM) files obtained by mapping the paired-end read sequences to the human reference genome using BWA [ 24] (or any other alignment tool that can produce BAM files).
First, MISO defines p k differently than IUTA does, using where μ is the mean of the empirical fragment length distribution f' that is obtained by mapping all paired-end reads to long constitutive 3′ UTRs (UnTranslated Regions).
Span size is the distance (in base pairs) on the reference sequence spanned by discordantly mapping read pairs.
In contrast to all other available programs, ReadXplorer classifies the read mapping data during the import process by mapping quality measures and read pair concordance.
An optional mode can increase stringency by ensuring that at least one read of a read pair maps uniquely within reference the genome.
TRIP [ 3] uses an integer programming model where the objective is to select the smallest set of putative transcripts that yields a good statistical fit between the fragment length distribution empirically determined during library preparation and fragment lengths implied by mapping read pairs to selected transcripts.
The data set was reduced as above to remove additional read-pairs mapping in the same location leaving 2,808 Class 2 events that were indicated by a single read-pair.
SVs were tested based on the expectation that authentic SVs would be supported by abnormally mapped read pairs, and that deletions with respect to the reference should have lower mapped read depth than average [ 20, 22, 23].
