DOI: http://dx.doi.org/10.7554/eLife.07789.013 Average speed of sporozoites was determined by manually tracking sporozoites throughout the duration of video, which includes periods of stopping and phases of continuous movement.
Using video and image analysis techniques, surface velocity data measured with a Visiofroth system were validated by comparing those results with estimations from manually tracking the motion of the bubbles on the froth surface.
The recorded data were analysed with ImageJ software either by manually tracking filaments or by tracking barbed ends using the plugin MtrackJ.
The mean winds over our analysis period (December 2015 March 2017) obtained from the 365-nm UVI images were compared with the mean winds obtained by manually tracking VEx/VIRTIS images at 365-nm from April 2006 to February 2012 (Hueso et al. 2015).
Assembly and disassembly was quantified by manually tracking leading and trailing edges of FAs using the MTrackJ plugin for ImageJ™.
Images used to manually track the bubbles were recorded using a high-speed video camera.
Cell motility was quantified manually, tracking ≥35 cells/condition throughout the time stack, using the ImageJ software.
Specifically in its current implementation, it requires manually tracking of landmark cones through the volume video.
Individual cells were then manually tracked using the ImageJ™ cell tracking plugin, and the speed of random migration was calculated in μm moved/hour.
