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Velocity-time integral (VTI) was measured by manually tracing the flow envelope for each image (Fig. 1).
Each region was determined by manually tracing the shape of BF images in the same field of view.
MVO was quantified by manually tracing the central hypoenhanced area and expressed as a percentage of LV mass.
The TIV was computed by manually tracing the entire intracranial cavity on 7 mm thick coronal slices, by the use of the software DISPLAY 1.3 tools.
Analysis of neurite length was done by manually tracing the neurites using Metamorph software (Universal Imaging Corporation, West Chester, PA, U.S.A).
The largest tumor area in a coronal plane was measured by manually tracing the margin of the tumor using Vevo 770 software.
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By manually tracing these 34 contigs to the genome scaffolds, we found that they were distributed within 18 scaffolds (Table 4), apparently widely dispersed in the genome of S. japonicum as inferred from the genome map.
The tibia (MT) and central weight-bearing medial femur (cMF) are segmented by manually tracing of the total area of the subchondral bone (tAB) and the area of the cartilaginous joint surface (AC) on a slice-by-slice basis [ 36, 37].
Corpus callosum area was derived by manually tracing around the edges of the corpus callosum.
In order to provide a quantitative assessment of the various automated methods' performance, we produced a gold standard segmentation by manually tracing all the visible retinal vessels in each of the twenty VIO images.
For each vibratome section, a two-dimensional projection of AP-labeled neurites was obtained by manually tracing over the electronic images using an electronic stylus (Wacom, Saltama, Japan) and Illustrator CS2 software.
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