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SNP validation was done by manually checking the alignment and by PCR amplification and sequencing of the polymorphic sites in five genotypes.
Deletions predicted by DELLY were validated by manually checking the alignments and through PCR, by designing primers that bridge the putative gaps or that amplify from regions flanking the gap within the deleted region.
No insertion was automatically found in any of the sequences generated by the three platforms, including the CAAG duplication, ascertained with Sanger sequencing and by manually checking the UHTS alignments (dbSNP:5828571).
To obtain GO annotations for proteins without existing annotation, but between 70 90% amino acid sequence identity to presumptive orthologs with GO annotation were annotated using GOanna by manually checking the similarity between our proteins and orthologs [72].
Additional articles were identified by manually checking the reference list of included papers.
However, we confirmed the existence of its unacetylated form by manually checking the spectrum.
Similar(41)
To establish the list of functionally characterized genes in rice, we conducted a comprehensive search for articles related to rice functional genomics, and we extracted information on gene function by manually checking every article identified in the search.
The underlying quality of the person-based linkage in the WADLS has previously been measured by manually checking a sample of linked hospital admission and death records.
Huang et al. divided the sequences into"confirmed", "potential" and "non-transporter" by manually checking four annotations in SwissProt, i.e., protein names, gene names, function and sequence similarities.
Their citizenship of human gut bacteria were identified by manually checking their corresponding dossiers in Genome Project Database.
They subsequently validated their structured data by manually checking a random sample of these records.
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by manually editing the
by manually retrieving the
by manually screening the
by manually tabulating the
by manually opening the
by manually sizing the
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by manually measuring the
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