Sentence examples for by manually aligning the from inspiring English sources
Sequence alignment was accomplished by manually aligning the translated B- and A-chain conserved motifs and twin dibasic motifs, the latter correspond to the protease cleavage sites between the B/C and C/A chain.
Composites were manufactured by manually aligning the treated hemp bast fibre strips resulting in unidirectional composites.
This problem has been solved by manually aligning the anatomical terms from both repositories.
The number of introns and exons was determined by manually aligning the CDS sequences with the genomic sequences using ClustalW [ 72] and with the 'Gene Structure Display Server' (GSDS) web tool [ 73].
The teleost repeat sequence logo was obtained by manually aligning the individual 13 amino acid repeat units within exon 18=19 from D. rerio, locus 1 (6 repeat units) and locus 2 (13 repeat units), G. aculeatus, locus 1 (7 repeat units) and locus 2 (5 repeat units), T. nigroviridis, locus 2 (8 repeat units), and T. rubripes, locus 2 (6 repeat units), totaling 45 repeat units.
Specific elements were then identified manually by aligning the wallaby class I genes to Modo-UA, mammalian (pig) and non-mammalian (duck) class I promoters using ClustalW.
The sequences were manually aligned and the alignments were concatenated.
The sequences were manually aligned, and the position-specific frequency matrices were calculated by counting the occurrence of every nucleotide at each position.
All annotations were manually curated by aligning the contigs against the scaffolds of WB and GS using TBLASTX.
Families missing in one or the other genome were manually checked, by aligning the sequences of the family against the contigs of the genome where the family was missing.
Sequences were assembled with Sequencher (Gene Codes) and deposited at GenBank under the accession numbers listed in Additional File 3. Alignments for protein coding genes were generated with ClustalX [ 21] and were in some cases manually improved by aligning the amino acid sequence.
