Sentence examples for by manual dissection of from inspiring English sources

Pigmented cells were enriched by manual dissection of expanded colonies followed by dissociation in 0.05% Trypsin-EDTA.

Colonies were allowed to expand for a further 14 weeks before we enriched for pigmented cells by manual dissection of expanded pigmented colonies followed by cell dissociation.

Here, we collected cancer and benign tissues from the same individual prostate gland by manual dissection of frozen tissue for proteome profiling to avoid inter individual differential expression of proteins.

Undifferentiated hESCs were obtained by culturing them under conditions that have been shown to support the undifferentiated state, followed by manual dissection of colonies that morphologically appeared to be undifferentiated, and plating onto chamber slides for further growth and antibody staining.

All iPSC lines were passaged by manual dissection of cell clusters in the presence of 10 μM ROCK-inhibitor Y-27632 (Sigma-Aldrich, Buhs, Switzerland).

Therefore, direct sequencing requires the condensation of tumor cells by manual dissection of the tissue sections in which tumor cells are densely contained (manual microdissection).

Lungs were isolated by manual dissection with the aid of a dissecting microscope where necessary.

Cells were passaged by manual dissection every 5 7 days at a ratio of 1∶3 and medium was changed every 48 hours.

Cells were passaged by manual dissection every 4 6 days.

Single cells were isolated by manual dissection (HES2 at p36 42, eleven separate isolations) and also following separation by FACS (HES3 at p24 41, six separate isolations).

For isolation by manual dissection, small pieces were excised from mature (day 6) HES2 colonies from three regions (edge, mid, and adjacent to the center region).