To maintain the overall electrostatic neutrality conditions, Na+ ions were added to the simulated systems.
Accordingly, the Thr73Ala mutant effectively separates the electrostatic role of His197 from its additional roles in maintaining the chromophore environment and reveals that the electrostatic effect of this residue accounts for a blue-shift of at least 12 nm.
These results indicate that PEGylated ConA can overcome the instability issues from thermal denaturation and nonspecific electrostatic binding while maintaining the required sugar-binding characteristics.
Furthermore, since electrostatic interactions with the backbone on one strand are critical for maintaining the motor's grip on DNA, we reason that at least one electrostatic contact between the motor and the phosphate backbone is needed for SpoIIIE to maintain a stable grip on its DNA substrate.
This latter hypothesis proved to be correct because there is an electrostatic interaction between this residue and Arg343 that is presumably important for maintaining the overall structure.
By using a variation of salt concentration, the importance of electrostatic interaction is also found in KF. 48, 49 However, the authors of the above study 14 did not mention the amino acid residues interactions that play a role in maintaining the stability of the enzyme.
The stability of PEI-NH-CNT suspension may therefore be maintained by electrostatic repulsion contributed by the cationic PEI. Figure 7 Zeta potential of pristine and PEI-functionalized carbon nanotubes.
This interaction is additionally maintained by electrostatic interactions between the SH2 helix αA and kinase helix αC, which position and stabilize αC in an active conformation.
Dimer integrity is maintained by electrostatic interactions between charged amino acids across the dimer interaction surface.
The electrostatic repulsion of the linker DNA backbone maintains the chromatin fibre in an unfolded state.
