Sentence examples for by magnetic sorting using from inspiring English sources

The CD133− and CD133+ fractions were separated by magnetic sorting using the CD133 Microbead kit (Miltenyi Biotec, Auburn, CA).

GDTc were enriched from the PBMC fraction by magnetic sorting using the Miltenyi γδ T cell positive selection kit (Miltenyi Biotec, Auburn CA) as per the manufacturer's instructions.

Isolation of specifically desired cells was performed by magnetic sorting using the Miltenyi Biotec magnetic microbeads, MACS columns and MACS separator, according to the manufacturers instructions.

B cells were further isolated from these samples by magnetic sorting using CD19 labelled magnetic check beads (Milteny Biotech, Bergisch Gladbach, Germany).

Parasite lysate used in the in vitro phosphorylation assays was generated by separating infected [100 ml of synchronized 3D7 schizont culture with >7% parasitaemia (4% haematocrit)] from uninfected erythrocytes by magnetic sorting using VarioMACS (Militenyi Biotech).

In some cases, total RNA was extracted from either CD4 or CD8 T-cells enriched by positive magnetic sorting using an AutoMACS (Milteny Biotec).

Monocytes were isolated from buffycoats by Ficoll amidotrizoate density centrifugation and magnetic sorting using anti-CD14-coated beads (Miltenyi Biotec, Auburn, CA, USA) according to the manufacturer's instructions.

Porcine immature DCs were differentiated in vitro from blood monocytes isolated from PBMCs by immune-magnetic sorting using the Anti-CD172 monoclonal antibody and treated during 3 4 days with a mixture of recombinant porcine granulocyte-macrophage colony stimulating factor and interleukin 4 [NCP and FL, [23].

Highly pure CD8 T cell lines could be generated ex vivo by magnetic sorting by using HLA-B27 tetramers loaded with an EBV peptide.

NKT cells were enriched from adult PBMC or neonatal CD4+ T cells by staining with Vα24 antibody, followed by goat anti-mouse magnetic beads (Miltenyi Biotec), and positively sorted by magnetic cell sorting using AutoMACS (Miltenyi Biotec) as described [30].

The gonads were dissociated with 0.05% trypsin/0.02% DNase I (10 15 min, 37 °C), and endogenous PGCs were removed by magnetic cell sorting using anti-SSEA1 antibody conjugated with magnetic beads (Miltenyi Biotec) according to the manufacturer's protocol.