Sentence examples for by magnetic separation using from inspiring English sources
Spleen B cells were isolated by magnetic separation using CD19 microbeads (Miltenyi Biotec, Germany).
Monocultures of human CD56+, CD3+, CD14+ and CD19+ cells were obtained by magnetic separation using cell specific isolation kits (Milteny Biotec, Germany) following the manufacturer's instructions.
Finally we isolated CD2 cDNA from a cDNA expression library by magnetic separation using CD58-displayng BV and anti-gp64 antibody.
The cells were depleted of T cells by magnetic separation using anti-CD5 (Ox19) and anti-αβ T cell receptor (R73) antibodies conjugated to pan-mouse IgG coated Dynabeads (Dynal Biotech ASA, Norway).
Spleen B cells were isolated by magnetic separation using CD19 microbeads (Miltenyi Biotec, Germany) and then were lysed on ice for 15 min and centrifuged at 2,000 g for 5 minutes.
NK cells were isolated from splenocyte suspensions by magnetic separation using a Mouse NK Cell Isolation kit (Miltenyi Biotec).
Mononuclear cells were isolated from the heparinised blood samples by Ficoll Hypaque centrifugation followed by negative magnetic separation using the depletion technique (Miltenyi Biotech, Auburn, CA, USA) [ 4].
CD14+ monocytes were isolated from buffy coats (Centro Trasfusionale Brescia, Italy) by positive magnetic separation using CD14 immunomagnetic beads (Miltenyi Biotec, Auburn, CA) [60].
mRNA was subsequently recovered by a magnetic separation using Oligo dT 25 Dynabeads.
Alternatively, live CD4+ T cells were isolated by Ficoll centrifugation and then magnetic separation using the CD4+ T cell Isolation kit II and restimulated with either anti-CD3 and anti-CD28 coated beads (Dynabeads, Life Technologies) or on anti-CD3 (1 μg/mL, eBioscience; clone 145-2C11) and anti-CD28 (2 μg/mL, eBioscience; clone 37.51) coated plates.
