Sentence examples for by mRNA analysis for from inspiring English sources

Functional impact of methylation was evaluated by mRNA analysis for selected genes.

Exon skipping was confirmed by mRNA analysis for the two RAD51C mutations.

Induction of mediators of inflammation and destruction by a range of concentrations of 14-3-3η 14-3-3η 14-3-3ηml) were evaluated by mRNA analysis for interleukin 1β (IL-1β), IL-6, MMP-1, MMP-9, receptor activator of nuclear factor κB ligand (RANKL) and TNFα.

In this study, our aim was to follow a bioinformatic pipeline in order to identify autoimmune disease-associated variants with the potential to effect splicing, and to follow up interesting candidate variants by mRNA analysis in whole blood, an appropriate tissue for the analysis of autoimmune or inflammatory disorders.

By mRNA analysis G. graminis lox mRNA could be detected thus, it can be concluded the lox gene was successfully transcribed.

Transcriptional alterations related to HSF1 protein level were investigated by mRNA microarray analysis for 224 freshly frozen samples collected in parallel.

Cancer cells from the spleen of treated mice were isolated and purified by flow cytometry for mRNA analysis.

Tissue samples for mRNA analysis by microarrays or PCR were snap-frozen in liquid nitrogen in the operating room and stored at -70°C until analyzed.

For mRNA analysis by reverse transcriptase PCR (RT-PCR), 0.5 1 μg of total RNA was reverse-transcribed at 42°C for 60 min using 200 U/μL M-MLV (Moloney murine leukemia virus) reverse transcriptase (Promega, Madison, WI, USA) in the presence of oligo dT 12 18 primer (0.04 μg/μg of total RNA), 1 mM dNTP mix, and RNaseOUT ribonuclease inhibitor (1 U/μL; Invitrogen).

A fully automated method based on magnetic beads was applied for RNA extraction, followed by one-step quantitative RT-PCR for mRNA analysis of VEGF-A, -B, -C and vascular endothelial growth factor receptor (VEGFR) 1, 2, 3.

The isolated glomeruli were then used for mRNA analysis (see below) and protein analysis by homogenizing them in cell lysis buffer.