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Examination of NHERF1 expression in brain sections showed that NHERF1 is most highly expressed at the apical PM of ependymal cells, followed by lower expression levels in choroid plexus cells (Additional file 1: Figure S2).
Our studies revealed that while KCs are well equipped to respond to viral pathogens, latent infection with HPV results in suppression downstream of the PRRs as reflected by lower expression levels of effector molecules involved in innate and adaptive immune response.
Thus, correlation between LS and the rate of protein evolution is not entirely driven by lower expression levels of high LS genes.
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The absolute number of percentage of cells with Flag-S228P aggregates was lower than that of GFP-S228P, which might be caused the by lower expression level of Flag-S228P in the cells or inaccessibility of the Flag tag buried in the aggregates.
This result was not caused by lowered expression levels of RasGAP or Aurora B in RG27-expressing cells.
Altogether, our data indicate that SSQ1 was subjected to divergent evolution driven by lowered expression levels and functional diversification, but that critical function was maintained by purifying selection.
The metabolism of glucose and carbohydrates seems also to be hampered by early protein restriction as indicated by the lower expression levels of several genes involved in this metabolic pathway including lactate dehydrogenase C, UDP-glucose pyrophosphorylase, mannosidase alpha class 2A, malate dehydrogenase 2 and xylosyltransferase1.
The relative resistance of normal cells to TRAIL has been explained by the lower expression levels of functional death receptors relative to cancer cells [ 5, 6].
GSEA revealed significant enrichment (FDR = 0.006) of the lipid biosynthesis category by genes with lower expression levels in hibernating bears as compared to summer active animals.
Genes directly regulated by HIF showed lower expression levels (EPO, HPH1, HIF-1 alpha, HIF-1 beta, HIF-2 alpha, HIF-3 alpha, HPH1, HPH3, and EPOR) in the sea level children group than in both of the high altitude groups when their samples were collected at high altitude.
Correlating these different patterns of nucleosome occupancy with the expression levels of target genes indicated that genes with TFBSs exhibiting well-positioned nucleosomes on both sides or occupied by nucleosomes, had significantly lower expression levels.
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Justyna Jupowicz-Kozak
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