Our results demonstrated that TaqMan® PreAmp overcame the difficulties usually caused by low yields of RNA extraction from FFPE.
Photoautotrophic microalgae are a promising avenue for sustained biodiesel production, but are compromised by low yields of biomass and lipids at present.
However, our studies and those of others using in vitro mediated differentiation have been confounded by low yields of germ cells, inefficient meiotic progression and an incomplete imprinting status (8, 9).
These observation suggests that the magnitude of the Ca2+ release appears to be limited by the low yield of expression of the C-terminal fragment achieved by leaky ribosomal scanning of the second half of the fs-α1S message.
Practical applications of this method may currently be constrained by the overall duration of the procedures (∼24 hr total), and by the low yield of monodisperse vesicles.
The need for larger culture volumes was necessitated by the low yield of the protein after cleavage of tag.
Specifically, the "assembly" problem characterized by the low yield of unstable hybrid particles resulting from the insertion of foreign sequences and the "pre-existing immunity" problem due to the fact that the HBcAg is derived from a human pathogen have limited the development of this carrier technology.
Availability of artemisinin is limited by the low yield of A. annua and this is hindering availability of affordable malaria medicine to patients in developing countries.
Both systems have been limited to date by the low yield of tetrameric, active NA[12], [15] or a dependence on proteases to cleave the NA head from the membrane[16], [17].
Culture-based confirmation, however, is limited by the low yield of this method, due to a high susceptibility to contamination, e.g. after specimen preservation or transportation in sub-optimal conditions, or early antibiotic treatment [9].
