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Candidate vaccines to date have been hindered by low expression levels and the induction of non-neutralising antibodies.
In general, the technology is limited by low expression levels for nuclear-integrated transgenes, but recent progress in plant organelle transformation shows promise for enhanced expression.
The benefits of plants for the production of biopharmaceutical proteins include the ease of scale-up and the low pathogen burden, but the purification of target proteins is often affected by low expression levels and the abundance of host cell proteins in leaf extracts.
Primary adipocytes within the bone marrow are characterized by low expression levels of adipocyte-specific genes and high levels of genes associated with early adipocyte lineage.
The third and last cluster included three genes in family A and two genes in family P and was characterized by low expression levels.
However, after 5-Aza-2'-deoxycytidine and TSA treatment, there was an increase in expression of Cyclin D2 in T98G, but not in U87MG cell line (accompanied by low expression levels of the Cyclin D2 protein).
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The occurrence of only 58 candidates was verified in this study, which might be explained by low expression level of the remaining potential miRNAs in the mature cabbage leaves or their general low expression in the whole plant.
The lack of correlation for a minority of genes tested by qPCR may have been caused by their low expression levels and presence of multiple tags in all of them, a fact that has also been previously reported in other studies [e.g.,[ 45- 47]].
In this study, we show that AIRE promotes the expression of genes that can be characterized by their low expression levels and lack of transcriptionally active epigenetic modifications.
This was supported by very low expression levels for these transcripts observed in RISC-Free control samples 24 hours post IFNγ treatment in our second series of experiments.
Our data suggest that OA chondrocytes ex vivo are in a differentiation state similar to that of healthy chondrocytes, as shown by their low expression levels of ALK-1, similar expression levels of type I collagen, and high expression of type II collagens and aggrecan mRNA.
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by low serum levels
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by low sex levels
by low calcium levels
by low supply levels
by low staffing levels
by low cortisol levels
by low accumulation levels
by low light levels
by low glucose levels
by increased expression levels
by low payment levels
by low iodine levels
by similar expression levels
by actA expression levels
by high expression levels
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