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Articular cartilage is rich in collagen type II fibres and proteoglycans and is characterized by low cell density.
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Open raceway ponds are characterized by low cell densities up to 0.3 g L−1 [ 3].
The unique anatomical structure of articular cartilage is characterized by avascularity, low cell density and very dense extracellular matrix [ 1, 2].
Induction of TMEM45A expression was also observed when differentiation was induced by high calcium concentration at low cell density rather than by confluence and in vivo as shown by the immunohistological staining performed on human skin sections as indicated by a higher expression of TMEM45A in the upper layers of the epidermis.
Additionally, cytostatic effects of DDAVP were examined at low cell density by colony formation assay.
Both deadly CM and lactate caused loss of viability in low cell density, not preventable by either rapamycin or additional glucose.
The expression of cartilage proteins was more obvious on the surface of the scaffolds seeded with the high cell density than those seeded with the low cell density, as shown by the increased intensity in staining with Alcian blue/van Gieson and in staining for type II collagen (Fig. 5).
This can be explained by the high magnification objective lens used and low cell density.
Results from confocal laser scanning microscopy showed reduced CC chemokine receptor 1, but not CC chemokine receptor 5, expression by HaCaT cells at low cell densities, which was abolished in the presence of neutralizing antibodies against RANTES.
CAI-1 differs from HAI-1 and AI-2 by being detected at extremely low cell densities [22].
However, after several years of maintenance of the cell lines in the lab, we are unable to observe any repression of ferritin synthesis by IRP2 (or IRP1) at low cell densities (Fig. 5).
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