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The comparison step was performed by loading the array of 5 bp A. thaliana sequence positions into the next program, followed by the A. thaliana nucleotide-only sequence.
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Since actin depolymerization by Lat B inhibited endocytosis of FM4-64, the role of actin in maintaining an asymmetric array was investigated by loading the zygotes with dye prior to treatment.
Features and reporters are created by loading an array design file such as the gal file format used by GenePix [ 20].
Instead, we measured the variation indirectly by loading a droplet array of homogenous fluorescein solution (1 mg Fluka 46955 powder in 100 mL of water) and imaged the total fluorescent intensity of each droplet.
The algorithm described above needs to load the occurrence array O and the suffix array S in the memory.
Once cell loading was completed, beads were loaded into the array at high density to ensure that each well receives a single bead (>95% bead occupancy).
Next, sample cocktails were loaded onto the array and amplification was performed as recommended by the vendor.
After fragmentation Cy3-labelled samples were loaded on the array and hybridised over night (17h/65°C).
Samples were loaded onto the array and hybridized at 55°C for 20 hours.
The samples were then briefly spun down and filtered through a 0.45-micron durapore filter (Millipore), and 40 μl sample was loaded to the arrays by capillary force using a cover slip (Erie Scientific).
Detail analysis of wind loading on the array is provided in relation to the wind flow field surrounding the array.
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