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Selected fractions were collected and converted to the Trifluoroacetic acid (TFA) salt by loading on a preparative column as mentioned above and eluted using a linear gradient (slope 0.33 or %B/min) containing a mixture of solvents A [0.1% TFA/H2O] and B [0.1% TFA in CH3CN/H2O (75:25)] at a flow rate of 7.0 ml/min.
Recombinant LERP was further purified by loading on a 1 ml Anti-FLAG M2 affinity column (Sigma-Aldrich).
Light effects on polypeptides composition were also analyzed by loading on a SDS-PAGE samples at different stages of the light treatment.
The GST-Chi18H8 fusion protein was purified from the soluble fraction by loading on a GSTrap FF column 1 mL (GE Healthcare) using PBS at pH 7.4.
The samples were boiled for 5 min followed by loading on a 10 % SDS-PAGE gel (50ug pertein/10uL per well) for electrophoresis using a Bio-Rad mini gel apparatus at 100 V for 2 h.
OMPs were prepared following the procedure described by Onufryk et al.. Finally, the sample was suspended in 40 µL SDS buffer and a portion corresponding to 2.0 of600 of cells was analyzed by loading on a 10% polyacrylamide-SDS gel.
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Quantification and quality control were performed by loading one microliter of cDNA libraries on an Agilent DNA 1000 chip and running it on an Agilent Technologies 2100 Bioanalyzer.
The amplified libraries were pooled in equimolar amount and assessed by loading of a 1 μl aliquot on an Agilent Bioanalyzer 1000 DNA chip according to the manufacturer's instructions.
These could be used to exchange biomolecules by loading on transmembrane receptors.
These pollutants were positively correlated, underscored by their loading on a common factor, and had higher concentrations in winter than summer and inland compared to the coastal areas.
But Iran does not now have the ability to produce the fissile material to make a bomb, or to weaponize it by loading it on a missile — and, under the nuclear agreement, it won't for at least fifteen years.
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