Sentence examples for by linearizing the plasmid from inspiring English sources

The one adjacent to the EF1-NLS-βGal coding region was eliminated by linearizing the plasmid through a partial digest and blunt ending the vector and religating the blunt ends.

The Rorβ probes (kindly provided by Dr. M. Studer) were obtained by linearizing the plasmid containing the Rorβ coding sequence transcribed with either T7 RNA polymerase (antisense probe) or T3 RNA polymerase (sense probe) [17].

Digoxigenin labelled sense RNA for Xfz4-intronI was synthesizes by linearizing the plasmid with NotI and transcribing with SP6.

DIG-labeled par-1 antisense probe was made with the RE47050 cDNA clone by linearizing the plasmid with NotI enzyme and transcribing the cDNA insert with T3 polymerase.

Similarly, DIG-labeled camkII antisense probe was made with the IP15240 cDNA clone by linearizing the plasmid with EcoRI enzyme and transcribing the cDNA insert with Sp6 polymerase.

HeLa cells were cultured in DMEM (high glucose) with 10% FBS and 1% penicillin/streptomycin. HeLa cells stably expressing the non-silencing shRNA or Tat-SF1(C) were created by linearizing the plasmids with BstBI and ApaI, respectively, before transfection into HeLa cells using Lipofectamine 2000.

The plasmid was linearized with Apa I and transcribed with the T7 RNA polymerase for the antisense probe and the sense was generated with linearizing the plasmid with Sac I and the transcription was done with the T3 RNA polymerase.

Capped cRNAs encoding ion channels were synthesized after linearizing the plasmids and performing the transcription by a standard protocal [35].

Viral RNA was made by linearizing 10 μg of the plasmid with EcoRI, phenol chloroform extracting the DNA, and transcribing from the linearized DNA using the MEGAscript kit (Applied Biosystems, Foster City, CA).

After linearized by Bgl II, the plasmid pPIC9K- Sam2 was transformed into P. pastoris GS115 by the electroporation method with parameters: 1.5 kV, 200μF and 200Ω.

After the presence of the variant(s) was confirmed by sequence analysis, the plasmid was linearized and a TranscriptAid T7 high-yield transcription kit (Fermentas, Glen Burnie, MD) was used to transcribe the HCV genomic RNA from the plasmid.