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We present a four-dimensional simplified analytical expression of such hybrid map obtained by linearizing the uncontrolled impulsive hybrid nonlinear dynamics around a desired one-periodic passive hybrid limit cycle.
This is achieved by linearizing the "score" or the pdf approximate of the observation.
Theoretical impedance profiles were obtained by linearizing the model at every chosen membrane potential (see Materials and Methods).
Capped cRNAs encoding ion channels were synthesized after linearizing the plasmids and performing the transcription by a standard protocal [35].
Histogram equalization aims at linearizing the CDF.
The expression in Eq. (17) can be further simplified linearizing the hyperbolic arcsine term.
Linearizing the set of Eqs.
The idea of EKF is to linearize the non-linear function by approximating it with the first-order Taylor expansion.
Now, the active set strategy suggested by Grossmann and Floudas (1987) is used for linearizing this expression as: lambda_{ 1k} - alpha V_{ 1k} le 0, (36) s_{ 1k} - alpha ( 1 - V_{ 1k} ) le 0. (37).
The five Sac I restriction endonuclease linearized recombinant expression vectors were introduced into P. pastoris GS115 by electroporation using a Micropulser (Bio-Rad, USA) according to the manual, respectively.
Stable cell lines were generated by transfection of PvuI linearized expression vector pIRESneo containing cDNAs encoding GFP, V5- or GFP-tagged wild type ESCO2, or a GFP-tagged ESCO2 acetyltransferase mutant (W539G).
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