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The gradient used (flow rate of 1.0 mL/min) for all steroid metabolites starts at 90 : 10 (water : acetonitrile), followed by linear increase from 85 : 15 to 0 : 100 over 8 min, return to initial conditions and hold for 2 min; 10 min of total run length.
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Peptides were eluted by a linear increase from 5 to 28% acetonitrile in 0.1% formic acid over 130 min followed by a 55 min linear increase to 45% acetonitrile in 0.1% formic acid at a constant flow rate of 250 nl/min.
190 μg protein of each sample was loaded on the strip and IEF was performed on the Ettan IPGphor 3 IEF System (GE-Healthcare) in 4 steps for 9 h at 20°C applying the following program: the first step occurred at 500 V during 1 h, followed by a linear increase from 500 V to 1000 V over 4 h, 1000 V to 8000 V over 3 h and finally held 8000 V for 1 h in the final step.
IRI TEC shows a linear increase from 2009 to 2014.
The dawn transition consisted of a one hour linear increase from darkness to approximately 20% of the daylight level followed immediately by an increase to full daylight levels.
Coherent phonon transport is evidenced by linear increases of thermal conductivity with total superlattice thickness.
A linear gradient program was applied, increasing the mobile phase B from 30 to 50%% during the first 10.0 min, followed by a linear increase of mobile phase B up to 100%% from 10.0 to 10.2 min.
Hence, the increase in areal mass density was accompanied by a linear increase in softness.
By this definition, the so-called pause is seen to be meaningless because warming has continued apace, particular by the near linear increase in ocean heat content.
A study in the Netherlands observed a significant linear increase in AGW diagnoses from 2002 to 2007 (no P value provided), particularly those diagnosed by general practitioners [ 39].
When stimulated by Dex only, there was a linear increase in the rate of ApoE secretion from hMSCs.
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