Sentence examples for by linear gradient of from inspiring English sources

The C-terminal His6-tagged protein was subsequently eluted by linear gradient of 30 300 mM imidazole, using an Econo gradient pump system, at a constant flow rate of 1 mL min−1 and 1.0 mL fractions were collected.

After extensive washing using the lysis buffer, supplemented with 20 mM imidazole, the protein was eluted from column by linear gradient of 80 to 300 mM imidazole.

After injection of 100 µl of sample, adenine nucleotides were eluted by linear gradient of 5 mM ammonium dihydrogen phosphate at pH 3.2 and 750 mM ammonium dihydrogen phosphate at pH 4.0 at a flow rate of 1 mL/min for 50 min [49].

Proteins were separated by linear gradient of buffer B (25 mM Tris pH 7.4, 1 M NaCl) and characterized by immunoblotting with previously characterized monoclonal antibodies L8, 9D2, C4B10, or IST-7 [26] or antibodies 4D1 or 5C3 generated at the University of Alabama-Birmingham [27].

The precursors were purified by linear gradient of 20 60% B in 22 min, the desired compound eluted at 17 min. The resulting peptide solution was frozen by liquid nitrogen and lyophilized to afford purified precursors in fair yields (40 60%).

Following sample application peptides were separated by linear gradient (0.3 μl/min) of solution A (0.1% TFA/water) and solution B (80%ACN/0.11% TFA/water) for 60 minutes.

For analysis of purity and detection of fractions by UPLC MS throughout the isolation process, 5 μl was injected onto a UPLC MS system (Waters Corporation) and analysed by a linear gradient of 0.1% formic acid acetonitrile from 30%to50%0% over 3.4 min.

GG-SpaD protein was eluted by a linear gradient of buffer B (buffer A containing 400 mM imidazole), which was followed by size-exclusion chromatography using a Sephacryl S-200 (26/60) column (GE Healthcare) in buffer C (20 mM HEPES, pH 7.0, 150 mM NaCl, and 1 mM EDTA).

The volatile compounds are eluted first under isobaric conditions (1500 psi) in less than a minute followed by a linear gradient of the column back pressure (from 1500 to 3500 psi in 5 min) for the elution of the non-volatile compounds up to C40.

The supernatant was filtered (0.45 μm, Millipore), applied to a HisTrap™ HP (1 ml Ni-NTA column, GE Healthcare) equilibrated in 10 mM imidazole, 500 mM NaCl, 30 mM Tris/HCl, pH 8.0 and eluted by a linear gradient of 0 400 mM imidazole in the same buffer.

p11 was eluted by a linear gradient of (NH4 2SO4 from 50% to 0% and recovered in the last fractions.