Sentence examples for by linear gradient from from inspiring English sources

Elution was performed by linear gradient from 0.1 to 1 M NaCl.

The samples were eluted by 5% B for the first 5 min, and followed by a linear gradient from 5%to95%5% B over 50 minutes at the flow rate of 1 mL/min, and the column was maintained at room temperature.

Compound 10 (RT: 14.0 min; 2 mg) was isolated from another MPLC fraction (RT: 64.0 67.0 min; 1.8 g) by RP HPLC (linear gradient from 90 to 100%% B1 in 20 min, followed by 15 min isocratic conditions).

The column was eluted with water containing 0.1 % TFA (0 5 min), 40%% ethanol (5 10 min) followed by a linear gradient from 40 to 90%% ethanol in 5 min (flow rate 1 ml/min).

Samples were eluted at a flow rate of 0.5 ml/min with 0.05 M NaOH for 10 min followed by a linear gradient from 0 to 0.1 M Na-acetate in 0.05 M NaOH for 20 min and finally were eluted by 0.2 M NaOH and 0.5 M Na-acetate for 10 min.

Peptides were eluted from the column by acetonitrile linear gradient from 5 to 45 developed over 120 min at a flow rate of 50 mL/min.

After sample loading (10 mL, 6 mg/mL protein concentration), the column was washed with 10 volumes of buffer A (at 1 mL/min flow rate), followed by a linear gradient from 0.1 to 0.7 M NaCl over 210 minutes.

Protein elution was performed by a linear gradient from 0 to 300 mM NaCl.

Again protein elution was performed by a linear gradient from 0 – 500 mM NaCl.

This solution was applied on a Ni-NTA Superflow column (GE Healthcare) and eluted by a linear gradient from 0 to 500 mM imidazole.

Resolve the IPs by a linear gradient from 10 mM to 1.7 M ammonium phosphate (pH 3.5) for 25 minutes, followed by elution with 1.7 M ammonium phosphate for 20 minutes.