Sentence examples for by linear amplification using from inspiring English sources

Briefly, 2 ug of total RNA from each sample was used to generate double strand cDNA by linear amplification using oligo dT-T7 primer and reverse transcriptase.

Biotinylated cRNA target was prepared from the cDNA template by linear amplification using biotin-dNTPs and verified on a Bioanalyzer 2100.

Total RNA was labeled with Cyanin-5 CTP by linear amplification using a Low RNA Input Fluorescent Linear Amplification Kit (Agilent Technologies) as specified by the manufacturer.

The pellet was resuspended and total RNA was amplified by T7-based linear amplification using T7-oligo-dT-primers T7-oligo-dT-primers T7-oligo-dT-primers T7-oligo-dT-primers

Total RNA was isolated using PicoPure RNA Isolation Kit (Arcturus) and amplified by T7-based linear amplification using T7-oligo-dT-primers T7-oligo-dT-primers T7-oligo-dT-primers T7-oligo-dT-primers

Two μg of total RNA from each sample were amplified by doing two rounds of linear amplification using the Amino Allyl MessageAmp II kit (Ambion, CAT 1753).

Total RNA underwent one rounds of linear amplification using MessageAmp aRNA Kit (Ambion Inc. TX, USA).

Total RNA was subjected to one round of T7 based linear amplification using the amino Allyl Message Amp II amplification kit (Ambion) according to the manufacturer's protocol.

RNA extracted from glands was subjected to linear amplification using MessageAmp™ II aRNA Amplification Kit (Ambion).

Biotin-labeled cRNA was produced by linear amplification (AMIL1791; Ambion, Austin, TX USA) using 200 ng of quality-checked total RNA.

cell expression by linear amplification and sequencing.