The sections were evaluated by light microscopic examination, and cellular localisation and intensity (negative=0, weak=1, moderate=2, strong=3) of immunostaining in each section were assessed by two observers (RD and GIM).
To confirm the presence of Ewing sarcoma xenografts, lungs, kidneys, and macroscopically detected bone and soft tissue tumours were examined by light microscopic examination and by immunohistochemistry. Tissues were fixed in 4% buffered formalin, processed and embedded in paraffin.
Total leukocyte counts were obtained by light microscopic evaluation and the percentages of BAL fluid macrophages, neutrophils, eosinophils and lymphocytes were obtained by counting 500 leukocytes on randomly selected portions.
The level of expression was assessed by light microscopic visualization and scored as described previously by estimating the signal intensity (0 3) and the percentage of the tissue section staining positive (0 100%).
Cultures reached confluence after 6-7 dass, assessedsed by light microscopic examination, and were passaged at weekly intervals.
The percentage of neoplastic cells was estimated by light microscopic evaluation, and the samples containing a minimum of 60% dysplastic cells were chosen.
The sections were evaluated by light microscopic examination and the intensity of immunostaining in each section assessed independently by two observers (MM and GIM) using the scoring system described.
The zygote formation is recorded by light microscopic observations (Miyata and Miyata 1981).
Apoptosis was scored on a cell-positional basis by light microscopic analysis (H&E and TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling)) of 100 half-crypt sections per mouse with at least four mice in each group.
The cardioprotective nature of the GSP was further fortified by our light microscopic and ultra structural findings.
