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Previously, researchers have created pluripotent cell lines by isolating cells from early stage embryos; those cells are called ES cells.
Bone marrow dendritic cells (BMDCs) were prepared by isolating cells from the femurs and tibias of mice.
Generally, gene expression in growth plate chondrocytes is studied by immunohistochemistry, in situ hybridization or by isolating cells from various sources including cell lines and primary cell cultures; however, only a few studies have quantified global gene expression in the context of the whole growth plate or as a function of its constituent zones.
Microalgal cells in deionized water were prepared by isolating cells from the growth medium via centrifugation, washing and resuspending cells in deionized water.
hiPSCs can also be used to capture and study the phenotype of various genetic diseases [ 88] such as spinal muscular atrophy [ 89], Alzheimer's disease [ 90], familial dysautonomia [ 91], and Rett syndrome [ 92] by isolating cells from a patient harboring the genetic disease, creating an iPSC line, and differentiating that line to the cell type(s) affected by the disease.
This study uses a pure cell model, and eliminated genotypic variation by isolating cells from the same individual at different ages reducing much of the technical and biological variation that may have been present in previous studies offering a clear view of the age-dependent changes in TLR4 signaling.
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Preference for secreted compounds was measured by isolating cell-free supernatant from overnight cultures by centrifugation and inoculating 30 ul on either side of a 100 mm plate.
E14 chick metatarsal tendons were dissected and the surface cells isolated using a variation of the method described by Banes and co-workers to isolate cells from tendon by sequential enzymatic and physical release [16].
In this study we have attempted to characterise the cerebral microvasculature of schizophrenia patients by using laser microdissection to isolate cells from post mortem prefrontal cortex tissue.
Therefore, we investigated whether m909 recognizes these diseased cells by isolating such cells from two sources.
nTreg cells, corresponding to naturally occurring Treg cells defined by high FOXP3 expression, were generated by isolating CD25+ cells from UCB mononuclear cells as described previously [43].
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