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The switch from an LPAI virus to an HPAI virus phenotype is achieved mainly by introduction of multiple basic amino acid residues into the hemagglutinin cleavage site.
Certain genes which have no validated shRNA were knocked down by introduction of multiple shRNA sequences into cells to achieve better efficiency.
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This finding is an example of dengue outbreaks in the South Pacific Islands caused by introductions of multiple genotypes of the same DENV serotype into susceptible populations in the South Pacific Islands (2 ), in this instance, genotype I from Southeast Asia and genotype III from South America.
DOI: http://dx.doi.org/10.7554/eLife.05055.009 The BDSIR model assumes a closed epidemic, but the large-scale phylogenies generated using all available global data indicated that each of the annual epidemics were caused by the introduction of multiple viral lineages that went extinct locally by the end of the seasonal epidemic (data not shown).
Altering the freezing conditions by the introduction of multiple temperature gradients allowed collagen scaffolds to be produced with complex pore orientations, and anisotropy in pore size and alignment.
The in silico gene callers indicated many degraded genes/pseudogenes in these regions and careful examination of these fragments showed that they belonged to various classes of REs that have been inactivated by the introduction of multiple stop codons.
To facilitate cloning, the p166 vector was modified by introduction of a new multiple cloning site (MCS) between the BamHI and XbaI sites using linker ligation and primers: 5′-GATCCCCCGGGACCGCGGCAAGTCGACCAATCGCGAAAGGAATTCAAGTTAATTAAT-3′ & 5′-CTAGATTAATTAACTTGAATTCCTTTCGCGATTGGTCGACTTGCCGCGGTCCCGGGG-3′.
Conversion of the iodo compounds to the corresponding alkynes followed by cross-coupling with iodophenylindacenes catalysed by Pd(0) or with chloro-Pt II) chloro-Pt IItalysed by complexesbled the introducatalysedmultiple chromophore/luminophore units.
Introduction of multiple double-stranded breaks by endonucleases has the potential to induce intergenic alterations.
There's really nothing super new about it except the introduction of multiple screens.
The main modification consists in the introduction of multiple paths diffracted at the window edges.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com