Sentence examples for by introduction of expression from inspiring English sources

Here we describe a complementary approach using an innovative embryonic gut culture system in which the tubular architecture of the gut is maintained and rapid analysis of gene function can be achieved by introduction of expression constructs into the epithelial cell layer by using low-voltage square-wave electroporation.

Complimented strains of MN101 (JR32 lpg0634::aphA-3) and MN102 (JR32 lpg0901::aphA-3) were constructed by introduction of expression vector producing N-terminal M45 epitope tagged-Lpg0634 and Lpg0901, respectively.

Activation of EGF signaling pathways by the introduction of expression of transforming growth factor-alpha (TGFα) through a mammary epithelial cell-targeted transgene can interrupt the downregulation of STAT5 activation and promote mammary epithelial cell survival during involution [ 70].

Similarly, the ARE luciferase activity was increased by the introduction of expression vectors of Nrf2 or Jdp2 in WT MEFs and Jdp2 KO MEFs; however, the MafK repressed the ARE luciferase activity (Supplementary Figures S2c and d).

We therefore challenged their expression in patient samples and also explored iASPP inhibition in colorectal cancer cells by introduction of exogenous miRNA expression.

Transgenic plants were generated by introduction of the plant expression constructs into an Agrobacterium tumefaciens strain GV3101 and transformation was done by floral dipping (Clough and Bent, 1998).

Moreover, it must be determined whether transient gene silencing, as obtained by introduction of synthetic siRNA or expression plasmids, is sufficient for treatment, or whether the target gene must be silenced for an extended period of time by the use of viral expression systems.

In addition, blockade of JNK expression by introduction of a dominant-negative JNK into the cells lifted the inhibitory effect on the expression of c-FLIPL rendered by the treatment with PL or PL plus Dox.

The loss of function and mispairing could not be avoided by changing the TCR expression level or by introduction of an additional cysteine bridge.

Lentiviral expression was obtained by introduction of WT IRAK-M in the pHEF vector and virus production in 293T cells as described [ 39, 40].

Arsenite-induced Erk activation was markedly inhibited by introduction of dominant-negative Erk2 into cells, whereas expression of dominant-negative Erk2 did not inhibit JNKs or mitogen-activated protein kinase Erk kinase 1/2.