Sentence examples for by introduction and expression from inspiring English sources

To obtain evidence that the abnormalities in kidneys mentioned above are in fact caused by introduction and expression of a DT-A expression vector, we performed PCR and RT-PCR analyses.

Introduction and expression of these proteins by the virus into susceptible animals or animal cells in culture led to cell transformation.

Complimented strains of MN101 (JR32 lpg0634::aphA-3) and MN102 (JR32 lpg0901::aphA-3) were constructed by introduction of expression vector producing N-terminal M45 epitope tagged-Lpg0634 and Lpg0901, respectively.

Here we describe a complementary approach using an innovative embryonic gut culture system in which the tubular architecture of the gut is maintained and rapid analysis of gene function can be achieved by introduction of expression constructs into the epithelial cell layer by using low-voltage square-wave electroporation.

Localized introduction and transient expression of T-DNA constructs mediated by agro-infiltration of leaf tissues has been largely used in dicot plants for analyzing the transitivity and the cell-to cell movement of the RNAi signal.

We therefore challenged their expression in patient samples and also explored iASPP inhibition in colorectal cancer cells by introduction of exogenous miRNA expression.

Transgenic plants were generated by introduction of the plant expression constructs into an Agrobacterium tumefaciens strain GV3101 and transformation was done by floral dipping (Clough and Bent, 1998).

The introduction of prokaryotic expression elements and the precise design of the compatible RBS and Kozak sequence endow pOmni with pro/eukaryotic dual-expression capability, which was validated by cloning and expression of reporter gene EGFP in E. coli and HeLa cells in this study.

Similarly, the ARE luciferase activity was increased by the introduction of expression vectors of Nrf2 or Jdp2 in WT MEFs and Jdp2 KO MEFs; however, the MafK repressed the ARE luciferase activity (Supplementary Figures S2c and d).

Since neither growth suppression nor differentiation induction was observed by Xbp1U introduction, constitutive expression of Xbp1S could affect homeostasis of hematopoietic cells.

Activation of EGF signaling pathways by the introduction of expression of transforming growth factor-alpha (TGFα) through a mammary epithelial cell-targeted transgene can interrupt the downregulation of STAT5 activation and promote mammary epithelial cell survival during involution [ 70].