Sentence examples for by introducing the mutation from inspiring English sources

We tested for a functional effect by introducing the mutation (c.660C>T) into murine Adamtsl2 cDNA (R221C andMTSL2) and expressing the construct by transient transfection in three cell lines: HEK293F, COS-1, and CHO-K1 (Figure 3A C).

Because epithelial tumors of some tissues are frequently associated with Kras mutation and those of other tissues are rarely associated, we tested the hypothesis that some tissues are refractory to the effects of Kras mutation, as opposed to being susceptible to its effects but rarely undergoing its mutation, by introducing the mutation into multiple tissues.

The double mutant was obtained by introducing the mutation MUT2 in the pCdx2-1luc MUT1.

The mutant construct of ITGB1 3′UTR was obtained by introducing the mutation into the 7 nucleotides (GUGCCAUU) of the seed region for miR-183.

As an example, given an edge ϕ vw = 2, it can be either changed to ϕ vw ′ = 1 or ϕ vw ′ = 0. Mutations are allowed if the fitness of the individual improves by introducing the mutation.

A catalytic deficient version of the plasmid, A3B_Cat, was made by introducing the mutations E68A and E255Q (Burns et al., 2013).

To investigate the functional consequence of altering the conformational flexibility in the C-terminal tail of H4, we analyzed the effects of restricting the range of motion available to the H4 C-terminus on Asf1-dependent activities by introducing the mutations G94A and G94P to the tail of H4 and studied their effect in yeast and in vitro.

The generation of the S183/T185 double mutant occurred by introducing the T185 mutation into the S183-mutated cDNA.

We investigated this hypothesis by introducing the romR mutation into strains containing mutations that inactivated either the A-motility system (A−S+) or the S-motility system (A+S−).

A triple mutant where all three histidines were mutated to alanine was constructed by introducing the H306A mutation into the H252A/H232A double mutant.

By introducing the point mutation in embC, Goude et al. verified that the mutations D294G, M300L, and M300V increased susceptibility to EMB and that mutation M300I had no resistance effect [ 28].