The double mutant was obtained by introducing the mutation MUT2 in the pCdx2-1luc MUT1.
The generation of the S183/T185 double mutant occurred by introducing the T185 mutation into the S183-mutated cDNA.
SA is a crucial regulator of cell death shown by introducing the SA deficient mutant sid2 or a bacterial salicylate hydroxylase (NahG) into several lesion mimic mutants, including acd6, atg5, and dnd1[ 37, 46, 104].
We observed that the extended lifespans of Δsck2, Δpka1, and Δpyp1 mutants are all diminished by introducing the Δphx1 mutation, to similar or even lower levels than for the Δphx1 single mutant (Fig. 5).
Consistent with previous findings, complementing the oryR − mutant by introducing the oryR gene in the high-copy plasmid pBBROryR significantly decreased swimming as compared to WT bacteria, a phenomenon which is likely explained by the overexpression of oryR in a multicopy plasmid and the self-negative regulation of oryR [ 43].
Similarly, we depressed endocytosis in lin-10 pmk-3 double mutants by introducing the Pglr-1::rab-5(GDP) transgene.
Therefore, we generated two DNA binding deficient TWIST1 mutants by introducing the S144R/K145E and R118C mutations.
We tested the function of our binding mutants by introducing the proteins into an eco1-1 strain from which WPL1 had been deleted.
This reduction was further increased upon depletion of ARF4 activity by introducing the arf4-2 mutheion37 into the respective arf3 mutant backgrounds (Fig. 3c, d, g, h, m) although the primary stem conformation was similar as in wild type (Supplementary Fig. 7f-m).
After showing (for the first time) that heterozygote advantage leads to balanced polymorphism, he studied the survival of individual mutant genes by introducing the Galton Watson branching process and analysing it by functional iteration.
