Sentence examples for by introducing azide groups from inspiring English sources

In previous reports, GO-N3 was generated by introducing azide groups onto the carboxylic groups of GO through amidation with 3-azidopropan-1-amine (NH2(CH2)3N3) or via nucleophilic substitution using 2-chloroethyl isocyanate (Cl(CH2 2NCO).

We provide a simple method to introduce azide groups on GO sheets by the ring opening reaction of sodium azide with the epoxide groups of GO.

By introducing azide in this system, complex 2 forms the 2D network containing the [Co3] units.

By introducing propargyl group into the binaphthyl unit on the polyfluorene backbone, different Ir complex- and functional dendron-containing azides could be quantitatively clicked into the main chain through the Cu(I -catalyzed HuI -catalyzedpolar cycloaddition.

Next, we compared the labeling of substrate 2, 4 and BPA in HeLa cells followed by biotinylation of the introduced azides or alkynes via CuAAC (Fig.  6c).

Very recently, the reliability and robustness of the above described F-fluoroglycosylation strategy prompted us to extend the series of F-fluoroglycosyl azides by introducing 6-deoxy-6-[F]fluoroglucopyranosyl azide and 6′-deoxy-6′-[F]fluoromaltosyl azide (Scheme 2) [ 30].

A technique initially described by Gauchet et al. fulfills these criteria for soluble proteins by introducing a bioorthogonal azide or alkyne moiety that can be coupled to a complementary alkyne or azide on the surface by a Huigsen [3 + 2] cycloaddition reaction.

In a previous work, we developed a clickable boronic acid, 3- 2-azido-acetylamino phenylboronic acid (APBA) by introducing a terminal azide into commercially available 3- 2-azido-acetylamino phenylboronic

Lysates were prepared and the introduced azide-handles were biotinylated via CuAAC by incubating the lysate proteins (10 μg) with biotin-alkyne (0.1 mM) for 1 h at 4°C.

After fixation or cell lysis, the introduced azide- or alkyne-handles can be labeled via CuAAC, allowing detection by western blotting or fluorescence microscopy.

After azide groups were introduced on GO, alkyne groups were introduced via Steglich esterification of the preserved carboxylic groups with trimethylsilyl (TMS -protected propargyl alcohol, yielding double funcTMS -protectedor Click GO.